成果与论文

主要成就

本所自1994年成立以来，先后主持各类研究课题50余项。到2001年底到位研究经费820余万元，获得重点学科建设经费400余万元，获得技术转让和服务收入200余万元。其中包括:主持国家“863”项目两项、国家自然科学基金面上项目6项、国家教育部优秀青年教师基金项目3项、山西省自然科学基金项目12项、山西省科技厅攻关项目5项、山西省留学归国人员资助项目10项、山西省教育厅科技项目7项，获得横向科研与开发项目5项。

近5年来，本所共完成和发表研究论文100余篇，出版著作2部，通过省级科技成果鉴定5项，获得省级科技进步二等奖三项，获准和申报国家发明专利各一项。其中在国内外重要学术刊物《Gene》、《European J. of Cell Biol》、《 Bio tech - nology Letters 》、《 Journal of Bacteriology 》、《 Acta Horticulturae 》、《 Scientia Horticulturae》、《 Polyhedron 》、《 Chinese Journal of Biotechnology 》、《 Chinese Science Bulletin 》、《中国生物化学与分子生物学报》、《生物工程学报》、《动物学报》、《水生生物学报》、《实验生物学报》、《高技术通讯》、《植物病理学报》、《应用与环境生物学报》、《微生物学与免疫学杂志》等发表论文70余篇。

作为山西省生物化学与分子生物学及生物工程领域人才培养的重要基地， 1994年以来本所已毕业硕士生62名，培养博士研究生6名。

生物技术研究所2006年发表的SCI论文

序号	作者	联系人	论文名称	刊物名称	收录
1	赵军良	梁爱华	Transformation of modified cowpea trypsin inhibitor gene and anti-bacterial	Euphytica	sci 核心 (B 区 )
2	宋莉	梁爱华	Identification of transiational release factor eRFla binding sites on eRF 3 in Euplotes octocarinatus	Research in Microbiology	sci 核心 (B 区 )
3	付月君	梁爱华	Polyclonal antibody against a recombinant chlorotoxin-like peptide from the Chinese scorpion and detection of its putative receptors in human glioma cells	Biotechnology Letters	sci 核心 (B 区 )
4	任列香	杨斌盛	八肋游仆虫中心蛋白 N- 端半分子与 Tb3 , Ca2 的结合	无机化学学报	sci 外围
5	柴宝锋	梁爱华	Expression, characterization and immunolocalization of translation termination factor eRF 3 in the ciliate Euplotes octocarinatus	Research in Microbiology	sci 核心 (B 区 )
6	郝婵娟	梁爱华	Expression of an Insect Excitatory Toxin, BmK IT, from the Scorpion, Buthus martensii Karsch, and its Biological Activity	Biotechnology Letters	sci 核心 (B 区 )
7	王转花	王转花	Cloning,Expression,and Identification of Immunologicai Activity of an Allergenic Protein in Tartary Buckwheat	Biosci.Bioechnol.Biochem	sci 核心 (B 区 )
8	李玉英	王转花	Cloning and characterization of a novel trypsin inhibitor gene from Fagopyrum esculentum	DNA Sequence	sci 核心 (B 区 )
9	石亚伟	袁静明	Purification,enzymatic properties of a recombinant D-hydantoinase and its dissociation by zinc ion	World journal of Microbiology &Biotechnology	sci 核心 (B 区 )
10	孔荣	袁静明	酶分子在试管内的定项进化及其在手性有机物合成中的应用	化学进展	sci 外围
11	Martin	wolfang	Novel immunotoxin: A usion protein consisting of gelonin and an acetylcholine receotor fragment as a potential immunotherapeutic agent for the treament of Myasthenia gravis	Protein expression and purification	sci 核心 (B 区 )
12	刘晓秋	杨秀清	Cloning of transglutaminase gene from Streptomyces fradiaeand its enhanced expression in the original strain	Biotechnol lett	sci 核心 (B 区 )
13	李鹰奇	杨斌盛	Characterization of transferrin receptor-dependent GaC-Tf-FeN transport in human leukemic HL60 cells	Clinica Chimica Acta	sci 核心 (B 区 )
14	赵良启	赵良启	Sythesis of poly (3-hydroxybutyrate-co-3-hydroxyoctanoate) by a Sinorhizobium fredii strain	Letters In Applied Microbiology	sci 核心 (B 区 )

生物技术研究所历年发表的部分SCI收录论文

• Fabjan N, Wang Z H, Zhang Z, Kreft I. Tartary buckwheat as a source of dietary rutin and quercitrin. J. Agric. food Chem. 2003, 51,6452-6455.

• Ren W, Qiao Z, Wang H, Zhu L, Zhang L, Lu Y, Zhang Z, Wang ZH. Molecular basis of Fas and cytochrome c pathways of apoptosis induced by tartary buckwheat flavonoid in HL-60 cells. Methods Find Exp Clin Pharmacol. 2003, 25(6): 431-436.

• Wang Z H, Zhang Z , Zhao Z H, Gunilla W, Dan N and Ivan K. Purification and Characterization of a 24kDa Protein fro m Tartary Buckwheat Seeds. Biosci. Biotech. Biochemic. 2004,468(7):1409-1413.

• Wang Z H, et al. Cloning, Sequencing and Expression of Major Allergenic Protein Gene in Tartary Buckwheat. Biosci. Biotech. Biochemic.. 2006, 6 (in press) .

• Yuying LI, Zheng ZHANG, Aihua LIANG & Zhuanhua WANG* Cloning and characterization of a novel trypsin inhibitor (BTIw1) gene from Fagopyrum esculentum. DNA Sequance, 2006, 6 (in press).

• Zhao Z H, Wang Z H, Zhang Z, Gunilla W, Dan N. A comparative study of asthma, pollen, cat and dog allergy among pupils and allergen levels in schools in Taiyuan city, China and Uppsala, Sweden" Indoor Air 2006, 7 (in press) .

• Feng W, Shi Y , Li M, Zhang M. Tandem PDZ repeats in glutamate receptor-interacting proteins have a novel mode of PDZ domain-mediated target binding. Nat Struct Biol . 2003,10(11):972-8.

• Feng, W., Fan, J. S., Jiang, M., Shi, Y. W. , and Zhang, M. PDZ7 of glutamate receptor interacting protein binds to its target via a novel hydrophobic surface area. J Biol Chem 2002,277, 41140-41146.

• Guo C, Li Z, Shi Y , Xu M, Wise JG, Trommer WE, Yuan J. Intein-mediated fusion expression, high efficient refolding, and one-step purification of gelonin toxin. Protein Expr Purif . 2004 ,37(2):361-7.

• SHI,Y.W. , Zhao, L.X., Niu, L.X., Yuan, J.M. Gene sequence, soluble expression and homologous comparison of a D-hydantoinase from Pseudomonas putida YZ-26[J]. Chem.Comm.Chinese Uni. 2005, 21 (5) : 552-557

• Yawei SHI, Lixi NIU, X,FENG, Jingmig YUAN. Purification,enzymatic properties of a recombinant D-hydantoinase and its dissociation by Zinc ion[J]. World Journal of Microbiology and Biotechnology 2006, 22 (6) : 350-357

• Yawei SHI , Lifang CUI, Jingmig YUAN. Gene cloning,expression and substrate specificity of an imidase from the strain Pseudomonas putida YZ-26[J]. Current microbiology (2006,online)

• Martin Hossann, Zhuoyu Li, Yawei Shi , Ulrike Kreilinger,Pia D. Vogel, Jingming Yuan, John G. Wise, Wolfgang E. Trommer Novel immunotoxin: A fusion protein consisting of gelonin and an acetylcholine receptor fragment as a potential immunotherapeutic agent for the treatment of Myasthenia gravis[J]. Protein Expression and Purification 2006,46:73–84.

• Chai, B.F., Liang, A. et al., 2003, Agrobacterium-mediated Transformation of Kentucky bluegrass( poa pratensis L.) Acta Botanica Sinica. 8(45): 966-973 .

• Chai B.F. Liang, A. et al., 2003 Stable transformation of three cultivars of Kentucky bluegrass( poa pratensis L.) by particle bombardment of mature seed-derived highly regenerative callus. Agricultural Science in China.2(1):27-34

• Wang, W. B.F. Chai, A. Liang, 2004 ， Cloning, characterization and functional expression of the eRF1 gene of Blepharisma japonicum Biotechnology Letter, 26 （ 12 ）： 959-963 .

• Fu, Y.J., B.F. Chai, W.Wang, H. Zhi ， L.T. Yin, A. Liang ， 2004 ， Expression and Purification of the BmK Mm2 Neurotoxin from the Scorpion Buthus martensii Karsch and Its Biological Activity Test. Protein Expression and Purification, 38(1) ： 45-50 .

• Hao ， C.J. B.F. Chai, W. Wang, Y. Sun and A. Liang , 2005, Preparation of Polyclonal Antibody against Manduca Sexta Chitinase and Detection of Chitinase Expressed in Transgenic Cotton . Biotechnol Lett., 27(2):97-102. .

• Wang W., H. Zhi, B.F. Chai, A. Liang , 2005,Cloning and sequence analysis of the micronuclear and macronuclear gene encoding Rab protein of Euplotes octocarinatus. BBB, 69(3):649-652. .

• Zhi, H., W. Wang, L.Y. Li, B.F. Chai, Y.H. Sun, A. Liang . 2005, Cloning and Analysis of 16 Rab Genes from Macronuclear DNA of Euplotes octocarinatus. DNA sequence. 16(4):260-265 .

• He, X.J., Li, L., Hou, H., Zhao, Y., Liang A. Rao, Z., 2005, Purification, crystallization and X-ray diffraction analysis of centrin in Euplotes octocarinatus. , Protein & peptide Letter, 12(6):609-611.

• Fu, Y.J., B.F. Chai, W. Wang, L.T. Yin, A. Liang , 2005,Synthesization, Expression and Purification of a kind of Ameliorative Chlorotoxin from the Scorpion Buthus martensi Karsch and Its Biological Activity Test. Biotechnol Lett. 27: 1597–1603 .

• Hao, C.J., C.G. Xu, W. Wang , B.F. Chai, A.H. Liang , 2005, Expression of an insect excitatory toxin BmK IT from Buthus martensii Karsch and its biological activity test. Biotechnol Lett., 2005 27(23): 1929 - 1934 .

• Chai, B.F. W. Wang, and A. Liang, 2006 Expression,Characterization and Immunofluorescent Localization of Translation Termination Factor eRF 3 in Ciliates Euplotes octocarinatus. Research in Microbiology. 157:235-240..

• Zhao, J.L. B.D. Lu, H.L. Xu, M.H. Zhao, Z. Zhu , A. Liang. 2006, Genetic engineering of Chinese cabbage for insect resistance by CpTI Gene. Plant cell reports. (in press)

• Zhao ， J.L. H.L. Xu , Z. Zhu, A.H. Liang. 2006, Insect-resistant transgenic plant of Brassic pekinensis from Agrobacterium -mediated transformation of protoplasts. (in press)

• Fu YJ, Yin LT, A.Liang, 2006 Polyclonal antibody against a recombinant chlorotoxin-like peptide from theChinese scorpion and detection of its putative receptors in human glioma cells. Biotechnol Lett., (in press)

• Song, L. B.F. Chai, W. Wang, A. Liang 2006, Identification of translational release factor eRF 1a binding sites on eRF 3 in Euplotes octocarinatus. Research in Microbiology. (in press)

• 赵良启 肖婧凡冯涛王海滨 , Sythesis of poly (3-hydroxybutyrate-co-3-hydroxyoctanoate) by a Sinorhizobium fredii strain. Letters In Applied Microbiology. 2006(42)344-349.

• Martin 李卓玉石亚伟袁静明 , Novel immunotoxin: A usion protein consisting of gelonin and an acetylcholine receotor fragment as a potential immunotherapeutic agent for the treament of Myasthenia gravis. Protein expression and purification. 2006 ， 46,73-84

• Zhao, C.G. et al., Receptor-mediated Endocytosis of Transferrin in K562 Cells. Chinese Science Bulletin. 2003,48 （ 2 ）： 127-130.